Abstract
Purpose: To investigate the protective effect of Evodia rutaecarpa (Juss.) Benth. extract (ERBE) against Alzheimer's disease in 3xTg-AD mice.
 Methods: The cognitive function of 3xTg-AD mice was assessed using Morris water maze test. The levels of amyloid beta deposits and NeuN in the mouse hippocampus were evaluated by immunohistochemistry. Brain neurotrophic derived factor (BDNF) and tyrosine kinase B (TrkB) expressions were determined by western blot analysis.
 Results: ERBE treatment significantly ameliorated learning and memory deficits in AD mice, as shown by increased time spent in the target zone during probe tests. The escape latency in the animals treated with 400 mg/kg ERBE (20.5 ± 1.3 s) was significantly higher than untreated 3xTg-AD mice (12.4 ± 1.3 s, p < 0.01). In addition, ERBE significantly decreased Aβ deposits, increased NeuN-positive cells, and upregulated the expressions of BDNF (1.4 ± 0.2, p < 0.05) and TrkB (1.1 ± 0.2, p < 0.05) in 3xTg AD mice.
 Conclusion: The results suggest that ERBE administration may be a useful strategy for treating memory impairment induced by several neurodegenerative diseases.
 Keywords: Evodia rutaecarpa, Alzheimer, Memory impairment, NeuN-positive cells
Highlights
Alzheimer’s disease (AD) is the most common form of dementia in the elderly and accounts for between 50 and 75 % of all cases
We investigated to evaluate the neuroprotective effects of Evodia rutaecarpa (Juss.) Benth. extract (ERBE) on learning and memory deficits in a tripletransgenic mouse model of Alzheimer's disease (3xTg-AD), which expresses APPSwe, PS1M146V, and tauP301L [11]
It was demonstrated that ERBE treatment significantly increased the expression of hippocampal Brain-derived neurotrophic factor (BDNF) and tyrosine kinase B (TrkB). These results show that the induction of AD reduced BDNF and TrkB expressions in the hippocampus, whereas, ERBE treatment enhanced BDNF and TrkB expressions in the hippocampus of the 3xTg mice
Summary
Alzheimer’s disease (AD) is the most common form of dementia in the elderly and accounts for between 50 and 75 % of all cases. Alzheimer's disease is the most common form of dementia relating to memory and cognitive decline. There are a variety of drugs available for AD, such as cholinesterase inhibitors, glutamate receptor antagonists, and free radical scavengers These drugs do not closely target the pathogenesis of the disease and have numerous side effects [7]. Evodia rutaecarpa (Juss.) Benth., has been used in China in the treatment of various disorders, including stress-induced physiological changes, inflammation, hypertension, and cancer. Alzheimer's disease (3xTg-AD) mice carrying a mutant APP (KM670/671NL), a human mutant PS1 (M146V) knock-in, and tau (P301L) transgenes (B6; 129-Psen1tm1Mpm Tg (APPSwe, tauP301L) 1Lfa/J) were purchased from the Animal Research Institute, Nanjing University (Nanjing, China). The abstract ERBE was obtained by steeping the dried Evodia rutaecarpa (Juss.) Benth. Differences were considered statistically significant at p < 0.05
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
