Year-end Sale % OFF 
Abstract
Objectives Antinuclear antibody (ANA) positivity is a key finding in JIA-associated uveitis (JIAU), but there are quite a few patients with negative ANA. There is no relevant report on the difference of their clinical manifestations. Previous animal model studies have found that the occurrence of uveitis is related to macrophage activation. In this article, our goal is to investigate changes in the morphology and cytokines of peripheral blood mononuclear cells (PBMCs) in uveitis patients testing positive or negative for ANAs after lipopolysaccharide (LPS) stimulation. Methods A total of 30 patients were included in this study (10 in each group). They were divided into three groups (the ANA-positive [ANA+] group, ANA-negative [ANA-] group, and control group). There were ten patients (6 females and 4 males) in each group. Peripheral venous blood was collected into a heparinized tube, and PBMCs were isolated as soon as possible by the Ficoll-Hypaque density gradient separation method. Isolated cells were mixed with RPMI-1640 medium, and the cell concentration was adjusted to ensure that each patient had the same number of cells entering the study. After putting the extracted PBMC into the culture plate, LPS was added carefully to the plate. The cell culture supernatants were collected at 0 h, 3 h, 6 h, 12 h, and 24 h after LPS stimulation to detect the concentrations of IL-6, IL-1, TNF-α, and IL-10. Immunofluorescence was used to discover the deformation of macrophages after LPS stimulation. Results The newly isolated cells were approximately round. 6 h after LPS stimulation, the ratio of noncircular cells/circular cells was the highest in the ANA+ group. Unlike IL-10 that has been rising during the observation period, IL-6, IL-1, and TNF-α peaked at 6 h after LPS stimulation. Conclusion With LPS motivation, cytokines in the ANA+ group increased the most violently.
Highlights
Juvenile idiopathic arthritis (JIA) comprises a group of idiopathic arthritides that occur before the age of 16 years and persist for at least 6 weeks [1]
We found that most patients with an early onset age were antinuclear antibody (ANA) positive (ANA+)
After LPS stimulation, we found that part of cells changed in each group, and this mainly manifested as cell deformation and area enlargement
Summary
Juvenile idiopathic arthritis (JIA) comprises a group of idiopathic arthritides that occur before the age of 16 years and persist for at least 6 weeks [1]. With the significant improvement of diagnosis and treatment capabilities, the prognosis of JIAU has been greatly improved; the disease is still the main cause of visual impairment in children with uveitis [2]. We found that most patients with an early onset age were antinuclear antibody (ANA) positive (ANA+). This was consistent with Kotaniemi et al.’s finding that ANA positivity was a risk factor for early onset of JIAU [3]. Since the ocular symptoms were not obvious at the beginning of the onset, most of the discomfort cannot be accurately expressed by the children; some patients with initial uveitis were overlooked. It is imminent to explore the pathogenesis of this disease in order to obtain better treatment
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
