Effect of low-magnitude high frequency vibration on osteogenic differentiation of human periodontal ligament stem cells

Abstract

To investigate the effect of low-magnitude high frequency vibration (LMHFV) on proliferation, migration ability and osteogenic differentiation of human periodontal ligament stem cells(hPDLSCs). hPDLSCs were isolated from premolar and randomized into vibration culture group (magnitude：0.3 g; frequency：40 Hz; time：15 min/24 h) and static culture group. CCK-8 was used to identity the proliferation of hPDLSCs. Wound-healing assay was used to evaluate migration ability of hPDLSCs. The osteogenesis gene expression was analyzed by RT-PCR, and the osteogenesis protein expression was analyzed by Western blot. The osteogenesis differentiation capability was evaluated by alizarin red staining. The data were analyzed using SPSS 21.0 software package. After LMHFV, the proliferation and migration ability of hPDLSCs were increased. The expression level of RUNX2, ALP, Col-1, and OCN was significantly augmented under LMHFV. Alizarin red staining and Western blot proved the same trend. CONCLUSIONS：The results demonstrate that LMHFV can promote hPDLSCs proliferation, migration ability and osteogenic differentiation.