Effect of low-intensity therapeutic ultrasound on wound healing in rats subjected to third-degree burns

Rayanne Lisboa Mesquita,Jofre Jacob Da Silva Freitas,Wagner Coelho De Albuquerque Pereira,Aldo José Fontes-Pereira,Kathlen Oliveira De Oliveira,Pedro Iuri Castro Da Silva,Katia Simone Kietzer,Simone Haru Sawaki De Melo E Silva Silva

doi:10.1590/s0102-865020160010000006

Rayanne Lisboa Mesquita, Jofre Jacob Da Silva Freitas + Show 6 more

Open Access

https://doi.org/10.1590/s0102-865020160010000006

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Abstract

To determine the effectiveness of low-intensity therapeutic ultrasound (LITUS) on wound healing in rats with third-degree burns. Twenty rats were divided into the Control Group that comprised four rats without third-degree burns that did not undergo LITUS, the Burned Group (BG), comprising eight rats with third-degree burns that did not undergo LITUS, and the Burned with Treatment Group (BTG), comprising eight rats with third-degree burns that were administered LITUS. LITUS began 24 h after injury and involved daily applications for 8 min at 0.1 W/cm2 for 14 days. The BTG lost less weight than the BG (Q=2.75; p<0.05). No visible differences were apparent among the groups' lesions on day 4. By the end of treatment, wound healing was more evident in the BTG. No statistically significant differences were found between the BG and the BTG in relation to the parameters measured using the histological changes in burn wound healing scoring system. The LITUS protocol applied to the animals with third-degree burns accelerated the formation of fibrin-leukocyte crusts and significantly reduced weight loss. However, burn wound healing was not accelerated.

Highlights

Burns are a serious public health problem[1,2]
When comparing the groups together, there was a statistically significant difference among them in relation to weight loss [H(2) = 7.98; p = 0.018] (Figure2) and the post-test findings revealed that this difference was only between the Burned Group (BG) and Burned with Treatment Group (BTG) [Q = 2.75; p < 0.05]
The analysis of the coefficients of variation (CV) demonstrated that the BTG (CV = 0.06) had sustained less weight variation during the study compared with the BG (CV = 0.11) and the Control Group (CG) (CV = 0.07)

Summary

Introduction

Burns are a serious public health problem[1,2]. They represent one of the major causes of trauma in all age groups, and they lead to significant sequelae or even death. 4.5 deaths/100.000 people per year occur in low and middle income countries as a consequence of burns[2]. Large burns are one of the most aggressive causes of skin integrity losses. Burns are generally caused by flammable substances and overheated liquids[2]. Hypovolemic shock and infectious or inflammatory diseases can occur, which are among the most challenging situations that patients with burns and their care teams have to confront

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