EFFECT OF LOW TEMPERATURE STORAGE ON THE THERAPEUTIC PROPERTIES OF THE IMMOBILIZED ON ADSORBENTS AND FREE CELLS OF SACCHAROMYCES BOULARDII

Igor P Vysekantsev,Olga M Babinets,Valentyna F Martsenyuk,Nikolay V Repin,Igor F Kovalenko

doi:10.15414/jmbfs.2016.6.1.707-712

Igor P Vysekantsev, Olga M Babinets + Show 3 more

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https://doi.org/10.15414/jmbfs.2016.6.1.707-712

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Abstract

Probiotic S. boulardii cells were immobilized on adsorbents "Sorbex" (activated carbon pellets) and "SCMS-1" (granules of aluminum dioxide with deposited film of activated carbon). The experimental probiotics were the "carrier-cells" systems, wherein on the carrier surface different amounts of yeast cells were absorbed. The safety of the "carrier-cells" systems was assessed on the number of macrocolonies formed by certain systems. During freezing down to low temperatures bigger number of immobilized cells if compared to free cells in the cell suspensions has been shown to dye. The safety of the complexes during freezing is affected by cooling mode and preserving medium composition. Experimental intestinal dysbiosis was induced in mice by oral administration of ampicillin and metronidazole. There has been found a longer persistence of yeast cells and higher levels of Bifidobacterium spp., Lactobacilus spp. bacteria in the colon mucin of the mice treated with immobilized S. boulardii. In the groups of animals treated with free cells and the mixtures of free cells with adsorbents, the terms of persistence of yeast cells and the recovery rates of Bifidobacterium spp., Lactobacilus spp. in colon mucin were lower. Storage for one year at –80C, –196C did not affect therapeutic properties of immobilized and free S. boulardii cells.

Highlights

The "intestinal dysbiosis" term is considered as a symptom, testifying to quantitative and/or qualitative disorders in indigenous parietal intestinal microflora (Vorobyov et al, 1998)
The authors of research papers and patents dealing with the immobilization of probiotics on the carrires, determined the preservation of probiotic drugs either on cumulative effect on the composition of luminal intestinal microflora of the animals with experimental dysbiosis, or on the number of macrocolonies of probiotics after seeding by Koch dish method (Lusta and Fichte, 1990)
We used the conditions of low temperature storage of the immobilized on carboncontaining adsorbents S. boulardii probiotic ensured the safety of therapeutic doses required for a growth stimulation of parietal populations of Bifidobacterium spp., Lactobacilus spp. in mice with experimental intestinal dysbiosis

Summary

Introduction

The "intestinal dysbiosis" term is considered as a symptom, testifying to quantitative and/or qualitative disorders in indigenous parietal intestinal microflora (Vorobyov et al, 1998). Intestinal dysbiosis is an aggravating factor in clinical pathology of various origins and the functioning disorders of different organs and systems (Tkachenko and Suvorov, 2009). The range of therapeutic measures to correct the dysbiotic changes in the gut includes pathogenetic treatment of the main disease, if necessary it comprises a selective contamination of pathogenic and opportunistic microorganisms, restoring indigenous parietal intestinal microflora (Ardatskaya, 2008; Tokareva, 2011). The research aim was to study the effect of immobilized on adsorbents probiotic Saccharomyces boulardii, stored at low temperatures, on recovery of parietal microflora of Bifidobacterium spp., Lactobacilus spp. in the animals with experimental intestinal dysbiosis

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