Abstract
This study evaluated sperm viability over time, after dilution and refrigerator storage of fresh semen extended in either synthetic cauda epididymal plasma (CEP2), or in a low sodium medium (CJ2) supplemented with either AlbuMAX or egg yolk. Semen collected weekly for 4 weeks from 4 bulls and assigned within bulls, across treatments. After extension in either CEP2, or CJ2 containing either egg yolk or AlbuMAX, semen was cooled to 4°C, and evaluated for 7 days. A computer assisted sperm analysis (CASA) system was used for sperm evaluation. Particular emphasis was placed on sperm motility since it is the single most important sperm parameter influencing bull fertility. Total and progressive motility of sperm in CEP2 and CJ2-AlbuMAX were similar (P = 0.85 and P = 0.23, respectively), but both were lower (P < 0.01) when compared to CJ2-yolk. Fewer sperm had rapid motility in CEP2 and CJ2-AlbuMAX compared to CJ2-yolk (P < 0.01). Sperm straightness and linearity were greater in CJ2-AlbuMAX and CJ2-yolk than in CEP2 (P < 0.01). Mean velocity (VAP) and linear velocity (VSL) were greater (P < 0.01) in CJ2-AlbuMAX than either CEP2 or CJ2-yolk. The calculated curvilinear velocity (VCL) of spermatozoa in CEP2 was lower than CJ2-AlbuMAX (P = 0.01), but similar with CJ2-yolk (P = 0.54). Overall, every sperm parameter measured by the CASA system was equal to or higher for sperm stored 7 days in CJ2 medium as compared with CEP2. The CJ2 extender supplemented with egg yolk is a viable alternative for storing fresh bovine semen.
Highlights
50% of viable spermatozoa are detrimentally affected after exposure to either fresh or frozen storage procedures [1] [2]
Every sperm parameter measured by the computer assisted sperm analysis (CASA) system was equal to or higher for sperm stored 7 days in CJ2 medium as compared with CEP2
Spermatozoa straightness and linearity were similar (P = 0.48 and P = 0.06 respectively) in CJ2-AlbuMAX and in CJ2-yolk extenders; spermatozoa in CEP2 presented less (P < 0.01) straightness and linearity compared with spermatozoa of the other treatments
Summary
50% of viable spermatozoa are detrimentally affected after exposure to either fresh or frozen storage procedures [1] [2]. Studies had been conducted to define an optimal extender for storing fresh bull semen, and several have reported on extenders that improve sperm survival during prolonged storage [4] [5] [6] [7]. Few of these extenders prevented damage to the spermatozoa after dilution, resulting in eventual reduction in sperm quality parameters such as total and progressive motility after 5 or 6 d of storage [6] [8]. It might be beneficial to replace sodium chloride with choline chloride in sperm preservation medium
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
