Effect of low-level laser therapy on the membrane induced by the Masquelet technique at an orthotopic site in rabbits.

Jeniffer Gabriela Figueroa Coris,Sheila Canevese Rahal,Washington Takashi Kano,Maria Jaqueline Mamprim,Alexandre Botelho De Abreu Sampaio,Luciane Dos Reis Mesquita,José Ivaldo De Siqueira Silva Junior,Carlos Eduardo Fonseca-Alves,Letícia Rocha Inamassu

doi:10.1590/acb361003

Jeniffer Gabriela Figueroa Coris, Sheila Canevese Rahal + Show 7 more

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https://doi.org/10.1590/acb361003

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Abstract

ABSTRACTPurpose:To evaluate the low-level laser therapy (LLLT) on the membrane induced by the Masquelet technique in rabbits.Methods:Twelve Norfolk rabbits at approximately 3 months of age were used. A 1-cm segmental defect was induced in both radii, which were filled with polymethylmethacrylate cylinder. LLLT was used postoperatively in the bone defect of one of the forelimbs every 48 hours for 15 days. Six rabbits were euthanatized on third and sixth postoperative weeks.Results:In both forelimbs, radiographs showed new bone growth from radius cut ends on the third postoperative week and more advanced stage on the sixth postoperative week. Ultrasound showed induced membrane one week after the surgery. Histologically, there were no significant differences in the semi-quantitative score of inflammation intensity, total number of blood vessels, bone metaplasia, and collagen. The average thicknesses were 2,050.17 and 1,451.96 μm for control membranes and 2,724.26 and 2,081.03 μm for irradiated membranes, respectively, on third and sixth postoperative weeks. Vascular endothelial growth factor A (VEGF-A) and platelet derived growth factor (PDGF) expression were present in the induced membranes of control and irradiated forelimbs, but there was no significant difference.Conclusions:Based on assessment methods, it was not possible to demonstrate the effect of LLLT on the induced membrane.

Highlights

The reconstruction of bone defects is one of the greatest challenges in orthopedics, extensive segmental bone defects caused by high-energy accidents or debridement of infected bone fragments[1,2,3]
On the sixth postoperative week, bone growth from the cut ends was more intense in three control forelimbs (50%) and two forelimbs irradiated with level laser therapy (LLLT) (33.3%)
This study evaluated the influence of LLLT on the induced membrane, and there were no significant differences in histological characteristics compared to the control

Summary

Introduction

The reconstruction of bone defects is one of the greatest challenges in orthopedics, extensive segmental bone defects caused by high-energy accidents or debridement of infected bone fragments[1,2,3]. Various reconstruction techniques have been proposed, including bone grafting, bone lengthening, or bonetransport method, among others[2,4]. To ensure that the graft transferred to a bone defect be capable of performing its functions, studies have been conducted with synthetic polymer membranes[5], titanium cages[6], and Masquelet induced membrane technique[7]. The Masquelet induced membrane technique includes two surgical steps . 1,3,7,8 The first step comprises the site debridement, followed by the placement of the polymethylmethacrylate (PMMA) – based bone cement spacer in the bone defect[1,2,8]. The PMMA spacer prevents the interposition of soft tissue in the bone defect[7], promotes an inflammatory reaction, and induces the development of an encapsulation membrane[2]. The induced membrane is capable to prevent bone graft resorption and improves vascularity and corticalization[1,9]

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