Abstract
During cryopreservation, cells and tissue undergo dramatic transformation in chemical and physical characteristics. Thawing process is also an important step since the cryoinjury is not limited to the freezing process but may also occur during the thawing process. Laser by its photo-stimulation effect can improve the resistance to cooled storage of some human sperm and this will lead to improvement in the quality of frozen semen and in the potential of sperm fertilization. The objective of this study is to use laser as a method of thawing and its effect on human sperm motility and DNA integrity versus the thawing through room temperature. This prospective study carried on 70 cryopreserved semen samples. Each sample was prepared by centrifugation procedure, and divided into 2 parts, freezed and thawed by laser irradiation till melting for one part and by room temperature for other. The DNA integrity and sperm motility were assessed before vitrification and after the two methods of thawing. The results of cryopreserved semen samples showed that laser irradiation thawing has significantly increased sperm motility as well as a significant decreased DNA fragmentation (P< 0.05) versus room temperature thawing. Conclusion(s): Laser irradiation thawing of post freezing sperm improves post thaw motility and DNA integrity.
Highlights
Laser is a device that emits light through a process of optical amplification based on the stimulated emission of electromagnetic radiation
Laser irradiation by its photo-stimulation effect has been reported to improve the resistance to cooled storage of some sperm species [4] and this will lead to improvement in the quality of frozen semen, as well as it can improves the potential of sperm fertilization [5]
Sperm chromatin structure and DNA integrity are known to be altered or damaged during cryopreservation [10] were the cryopreservation process and thawing can reduce the ability of sperm chromatin to decondense and increase abnormal chromatin condensation in human sperm [11], in addition, cryopreservation could alter the quality of the spermatozoon’s nucleus [12, 13], induces DNA denaturation [14], increases sperm DNA fragmentation [15] and it causes liquid phase transition changes and increased lipid peroxidation leading to irreversible rupturing of plasma and nuclear membranes and disturbance of cellular organelles [16]
Summary
Effect of Low-Level Laser Irradiation Versus Room Temperature on Cryopreserved Sperm Motility and DNA Integrity as Modes of Thawing تاثير استخدام الليزر كطريقة لاذابة النطف البشرية المجمدة مقابل الاذابة بدرجة حرارة الغرفة على سلامة. Key wards: cryopreservation, laser irradiation, DNA integrity, sperm motility. فضلا عن طرق الاذابة التي تعد من,ان التجميد قد يؤدي الى تحولات مؤثرة في الخواص الكيميائية والفيزيائية للخلايا والانسجة المجمدة الخطوات المهمة والتي من الممكن ان تؤثر عليها ان اضرار الحفظ عن طريق التجميد لا تقتصر على اضرار التجميد فقط بل من الممكن ان تاثير التحفيز الضوئي لليزر من الممكن ان يحسن المقاومة لتاثير الحفظ عن طريق التجميد للنطف وهذا.ان تحدث اثناء الاذابة ايضا الغرض من الدراسة تاثير الليزر كطريقة.قد يؤدي الى تحسين جودة النطف المجمدة وبالنتيجة قد يؤدي الى تحسين القابيلة الاخصابية لها هذه الدراسة اجريت.لاذابة النطف البشرية المجمدة وتاثيرها على سلامة الاحماض النووية والحركة مقابل الاذابة بدرجة حرارة الغرفة واذيب كل جزء, جمدت, نموذج من النطف البشرية وبعد تحضيرالنموذج بطريقة الطرد المركزي قسمت النماذج الى قسمين70 على درس تاثير التجميد والاذابة بالليزر وبدرجة حرارة الغرفة على سلامة الاحماض. حركة النطف, سلامة الاحماض النووية, الاذابة بالليزر, التجميد بطريقة التزجيج:الكلمات الدالة
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
