Abstract
BackgroundQuality bone regeneration, which leads to the improvement of bone remodeling, is essential for orthodontic treatment. In order to improve bone regeneration and increase the amount of tooth movement, different techniques have been implemented. The object of this study is to compare the effects of low-level laser therapy (LLLT), low-intensity pulsed ultrasound (LIPUS), and their combination on bone remodeling during orthodontic tooth movement.MethodsEighty (80) male, 6-week-old Sprague Dawley rats were grouped in to four groups, the first group was irradiated with (940 nm) diode laser, second group with LIPUS, and third group with combination of both LLLT and LIPUS. A forth group used was a control group in an incomplete block split-mouth design. The LLLT and LIPUS were used to treat the area around the moving tooth once a day on days 0–7, then the experiment was ended in each experimental endpoint (1, 3, 7, 14, and 21 days). For amount of tooth movement, models were imaged and analyzed. Histological examination was performed after staining with (hematoxylin and eosin) and (alizarin red and Alcian Blue) stain. One step reverse transcription-polymerase chain reaction RT-PCR was also performed to elucidate the gene expression of RANK, RANKL, OPG, and RUNX-2.ResultsThe amount of tooth movement, the histological bone remodeling, and the RT-PCR were significantly greater in the treatment groups than that in the control group. Among the treatment groups, the combination group was the highest and the LIPUS group was the lowest.ConclusionThese findings suggest that LLLT and LIPUS can enhance the velocity of tooth movement and improve the quality of bone remodeling during orthodontic tooth movement.
Highlights
Quality bone regeneration, which leads to the improvement of bone remodeling, is essential for orthodontic treatment
These findings suggest that level laser therapy (LLLT) and low-intensity pulsed ultrasound (LIPUS) can enhance the velocity of tooth movement and improve the quality of bone remodeling during orthodontic tooth movement
The comparison at day 7 showed that all the treatment groups were significantly higher than the control group, but there was no significant difference between the treatment groups
Summary
Quality bone regeneration, which leads to the improvement of bone remodeling, is essential for orthodontic treatment. The object of this study is to compare the effects of low-level laser therapy (LLLT), low-intensity pulsed ultrasound (LIPUS), and their combination on bone remodeling during orthodontic tooth movement. In vivo rat experiments, LLLT stimulated midpalatal suture bone regeneration during expansion [9] and increase the tooth movement [4, 5, 10], and LLLT irradiation improves connective tissues turnover with bone remodeling process acceleration by stimulating osteoblast and osteoclast cell proliferation and function, such as elevating the ALP and RANK/ RANKL/OPG system during orthodontic tooth movement [6, 11,12,13,14]. In vivo human studies have shown tooth movement acceleration, significant reduction in levels of pain during orthodontic treatment, and healthy response from periodontal tissues after LLLT irradiation [15]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
