Effect of low-level helium–neon laser therapy on the healing of third-degree burns in rats

Abstract

This paper presents the results of a study on the effects of low-level helium–neon laser therapy (LL He–Ne LT) on the healing of burns. Seventy-eight adult male rats, having been subjected to third-degree burns, were randomly divided into four groups: two laser treated groups ( n = 20, each), one control group ( n = 19) and one nitrofurazone treated group ( n = 19). In the two laser treated groups, the burns were treated on a daily basis with LL He–Ne LT with an energy density of 1.2 and 2.4 J/cm 2, respectively. The response to treatment was assessed histologically at 7, 16 and 30 days after burning, and microbiologically at day 15. Analysis of variance showed that the mean of blood vessel sections in the 1.2 J/cm 2 laser group was significantly higher than those in the other groups and the mean of the depth of new epidermis in the 2.4 J/cm 2 laser group on day 16 was significantly lower than in the nitrofurazone treated group ( P = 0.025, P = 0.047, respectively). When Staphylococcus aureus and Pseudomonas aeruginosa grew in more than 50% of samples obtained from control group, there were no S. aureus and P. aeruginosa in the samples of 2.4 J/cm 2 laser group. It is concluded that LL He–Ne LT induced the destruction of S. aureus and P. aeruginosa in third-degree burns of rats, yet at the same time our histological findings showed that LL He–Ne LT caused a significant increase in the mean of blood vessel sections on day 7 after third degree burns and a decrease in the mean of the depth of new epidermis on day 16 after the same burns in rats.