Effect Of Low Intensity Eccentric Exercise Training On Maximal Eccentric Exercise İnduced Muscle Damage

Abstract

PURPOSE: The aim of this study was to investigate the effects of low-intensity eccentric exercise performed before maximal eccentric exercise which causes muscle damage and oxidative stress on muscle injury markers and oxidative stress. METHODS: The study was carried out with 22 male Wistar albino rats obtained from Experimental Animal Research and Application Center of Selcuk University. Rats were randomly divided into 3 groups. Rats in the control group (K, n: 6) were not given any exercise protocol. The maximal eccentric exercise protocol was applied to the rats in the maximal eccentric exercise group (M, n: 8) only one at rate of 20 m / min, at -150 inclination for 90 min. Low intensity eccentric exercise protocol, which does not cause muscle damage was applied to the rats in the low intensity eccentric exercise + maximal eccentric exercise group (DY+M, n: 8) at 10 m / min, at -150 inclination, 30 min / day for one week and maximal eccentric exercise protocol was applied 24 hours after this exercise. Blood and muscle tissue samples were taken 24 hours after the last exercise session and creatine kinase (CK), lactate dehydrogenase (LDH) and superoxide dismutase (SOD1) activities and myoglobin (Mg), malondialdehyde (MDA) and glutathione (GSH) levels were measured. RESULTS: Serum Mg level was higher in the M group compared to the DY+M and the K groups. Serum CK and LDH activities were higher in the DY+M and the M groups compared to the K group. MDA level was higher in M group compared to K and DY+M groups in vastus intermedius muscle, in M group compared to the K group in gastrocnemius muscle and in the DY+M and the M groups compared to the K group in plasma. GSH level in the vastus intermedius muscle and plasma was higher in the DY+M group compared to the M group. GSH level in gastrocnemius muscle was not different between groups. SOD1 activity was higher in the DY+M group compared to the K group in the vastus intermedius muscle, in the DY+M group compared to the K and the M groups in the gastrocnemius muscle. SOD1 activity in plasma was not different between groups. CONCLUSIONS: Low intensity eccentric exercise training applied before the maximal eccentric exercise has a partial protective effect against muscle damage, especially in the vastus intermedius muscle prevents the formation of oxidative stress and strengthens the antioxidant defense.