Effect of long-term L-dopa administration on the dopaminergic and cholinergic (muscarinic) receptors of striatum in 6-hydroxydopamine lesioned rats

Abstract

L-Dihydroxyphenylalanine (L-Dopa) (200 mg/kg/day) was administered for 30 days to the rats whose nigrostriatal dopamine pathway was lesioned unilaterally with 6-hydroxydopamine and the receptor binding of 3H-spiperone and 3H-quinuclidinyl benzilate ( 3HQNB) was measured in the dopaminergic and muscarinic cholinergic receptors of the striatum. 3H-spiperone binding increased by 73% and 3HQNB binding decreased by 14% in the lesioned side when compared to the control side of L-Dopa-non-treated rats. 3H-spiperone binding was measured in the lesioned sides of L-Dopa-treated and L-Dopa-non-treated rats and was found to have decreased by 21% in the former. In the control side of the L-Dopa-treated lesioned rats, however, 3H-spiperone binding increased by 27% when compared to the opposite striatum of the same rats. 3HQNB binding in the lesioned side of L-Dopa-treated rats was not significantly different from that of the control side statistically. These results suggest that changes in functional equilibrium between the dopaminergic and cholinergic mechanisms influence the muscarinic cholinergic receptors and that supersensitivity of dopamine receptors after lesion of the nigrostriatal pathway also remains after long-term L-Dopa treatment.