Effect of Long-Term and Short-Term Droughts on the Hydrolytic Enzymes in Haplic Luvisol

Abstract

The transformation of organic matter in the soil is largely determined by hydrolytic enzymes. Under the conditions of climate change, understanding the mechanisms of microbial response is of particular importance for predicting the carbon cycle. Until now, the effect of drought duration and frequency on soil hydrolytic enzymes has been little studied. A multifactorial field manipulation experiment was carried out, simulating in the presence of plants and without them: two short-term droughts, a long-term drought, and an optimal level of soil moisture. The maximum reaction rate Vmax, Michaelis constant Km, and catalytic efficiency Ka of five groups of enzymes involved in the carbon cycle (cellobiase, glucosidase, xylanase), phosphorus (phosphatase), and nitrogen (chitinase) were determined. In phosphatases, glucosidases, and xylanases, Vmax decreased during short-term drought. During prolonged drought, the Vmax value of phosphatases, cellobiohydrolases, and xylanases decreased and increased in chitinases, while remaining unchanged in glucosidases. Both long-term and short-term droughts led to an increase in Km and a decrease in catalytic efficiency (Ka) for almost all enzymes. Short-term droughts were not a “weakened version” of a long-term drought, but had their own specifics – a decrease in Km in glucosidases, which led to an increase in Ka. Long-term drought was characterized by an increase in Vmax of chitinases and spatial variability of Vmax of phosphatases and glucosidases. The influence of the presence of plants was secondary and affected only during short droughts. The reversibility of the effect of drought on Vmax, Km, Ka decreased in the series first short-term drought second short-term drought long-term drought due to an increase in the total duration of the stress impact.