Effect of long non-coding RNA RGD1566401 on apoptosis of rat pancreatic acinar cells in the rat acute pancreatitis model

Abstract

Objective To analyze the expression of RNA-RGD1566401 (long non-coding RNA-RGD1566401, lncR-RGD1566401) in rat pancreatic acinar cells (AR42J) and the effect of lncR-RGD1566401 on apoptosis of AR42J cells. Methods The GFP LncR-RGD1566401 overexpressed lentivirus and special long non-coding RNA (lncRNA) Smart Silencer were designed and transfected into AR42J cells repectively for 24 h. The apoptosis of AR42J cells in different groups was established by inducing cells with 100 nmol/L Caerulin for 24 h. The flow cytometry was used to detect the apoptosis of AR42J cells in different groups. Real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) was used to detect the expression level of lncR-RGD1566401 in AR42J cells of different groups after transfection. The expression of cysteinyl aspartate-specific protease (Caspase)-3 and p53 was detected by Western blotting. Results The apoptosis rate [(19.34±0.54)%] of Caerulin-induced AR42J cells was elevated as compared with the control group [(7.23±0.33)%]. The expression of lncR-RGD1566401 in the AR42J cells was increased by (5.43±0.31) times in the experimental group after treatment with Caerulin and the levels of Caspase-3 and p53 were increased obviously. LncR-RGD1566401 level was elevated by (8.24±0.22) times after AR42J cells were transfected with lenti-lncRNA as compared with the vehicle groups (P=0.006). The AR42J cells showed more tendency to apoptosis [(38.20±0.37)%] than the vehicle groups [(22.60±0.96)%, P=0.016] and the levels of Caspase-3 and p53 were increased obviously. The AR42J cells that were transfected with Silencer-lncRNA expressed the lower level of lncR-RGD1566401 about [(0.54±0.14), P=0.003] times and less apoptosis [(13.60±0.33)%] than the Silencer-NC groups [(19.60±0.46)%, P=0.012]. Conclusion LncR-RGD1566401 is associated with the apoptosis of AR42J cells, and up-regulation of LncR-RGD1566401 can promote the apoptosis of AR42J cells. Key words: Long non-coding RNA; Apoptosis; Pancreatic acinar cells