Effect of long chain unsaturated fatty acids on the calcium transport of sarcoplasmic reticulum

Abstract

The effect of archidonic, oleic and linoleic acid on calcium uptake and release by sarcoplasmic reticulum isolated from longissimus dorsi muscle was investigated using a Ca 2+ electrode. All three long chain fatty acids stimulated the release of Ca 2+ from sacroplasmic reticulum when added after exogenous Ca 2+ was accumulated by the vesicles, and also inhibited Ca 2+ uptake when added before Ca 2+. This inhibitory effect on the calcium transport by arachidonic, oleic and linoleic acid was prevented by bovine serum albumin through its ability to bind with the fatty acid. The order of effectiveness of the fatty acids in inhibiting calcium transport by isolated sarcoplasmic reticulum was arachidonic acid> oleic acid > linoleic acid. Similar inhibition of calcium uptake and induction of calcium release by arachidonic acid was observed in muscle homogenate sarcoplasmic reticulum preparations. Both arachidonic and oleic acid stimulated the (Ca 2+ + Mg 2+)-ATPase activity of sarcoplasmic reticulum at low concentrations, but inhibited the (Ca 2+ + Mg 2+)-ATPase activity at high concentrations. The maximal (Ca 2+ + Mg 2+-ATPase activity observed with arachidonic acid was twice that obtained with oleic acid, but the concentration of arachidonic acid required was 3–4-times greater than that of oleic acid. The concentration of arachidonic acid required to give maximum stimulation of the (Ca 2+ + Mg 2+)-ATPase activity was 3.6-times greater than that needed for complete inhibition of calcium accumulation by the sacroplasmic reticulum. With oleic acid, however, the concentration required to give maximum stimulation of the (Ca 2+ + Mg 2+)-ATPase activity inhibited the sarcoplasmic reticulum Ca 2+ accumulation by 72%. The present data support our hypothesis that, in porcine malignant hyperthermia, unsaturated fatty acids from mitochondrial membranes released by endogenous phospholipase A 2 would induce the sarcoplasmic reticulum to release calcium (Cheah K.S. and Cheah, A.M. (1981) Biochim. Biophys. Acta 634, 70–84).