Effect of local high fatty acid and high carbon acid on tumor calcification and growth in an HCC827 xenograft model of human lung adenocarcinoma

Abstract

Objective To investigate the effect of local high fatty acid and high carbon acid on tumor calcification and growth in an HCC827 xenograft model of human lung adenocarcinoma. Methods Forty HCC827 human lung adenocarcinoma-bearing nude mice with an average tumor diameter of 10-15 mm were chosen and randomly divided into four groups to receive intratumor injection of oleic acid alone, sodium bicarbonate alone, oleic acid plus sodium bicarbonate, and normal saline, respectively. The changes of tumor volume and calcium deposition were then observed. Results HE staining results showed that obvious calcium salt deposition occurred in the oleic acid alone group and oleic acid + sodium bicarbonate group, but not in the sodium bicarbonate alone group and normal saline group, which suggested that local high fatty acid promotes tumor calcification. At the end of the experiment, the oleic acid alone group had the least tumor volume, followed by the oleic acid + sodium bicarbonate group, sodium bicarbonate alone group, and normal saline group, suggesting that oleic acid can inhibit tumor growth. Local high sodium bicarbonate inhibited tumor growth, but tumor volume continued to grow after stopping using sodium bicarbonate Conclusion Local high oleic acid can promote tumor calcium salt deposition, and oleic acid can inhibit tumor growth. Local high sodium bicarbonate inhibits tumor growth, which may be partially associated with changing the tumor acid environment by sodium bicarbonate. Key words: Oleic acid; Sodium bicarbonate; Calcification