Abstract

This study evaluated the effects of live yeast and yeast cell-wall mannan-oligosaccharide supplementation on performance and nutrient digestibility during early lactation in cows fed a diet based on a mixture of corn silage and alfalfa hay as forage sources. Eight multiparous Holstein dairy cows (average days in milk, 27±6) were used in a replicated 4×4 Latin square design. Diets contained 45% forage and 55% concentrate on a dry matter (DM) basis and treatments were: i) basal diet without additive (Control), ii) basal diet with 32 g/d of mannan-oligosaccharides (MOS), iii) basal diet with 1.2×10 10 colony forming units per day (cfu/d) of live yeast (Saccharomyces cerevisiae CNCM 1-1077; SC), and iv) basal diet with a mixture of MOS (32 g/d) and SC (1.2×10 10 cfu/d; MOS+SC). Treatments had no effect (p>0.05) on DM intake and yields of milk, 3.5% fat-(FCM) and energy-corrected milk (ECM), and on milk fat percentage, body condition score and blood metabolites. Compared with the Control, only supplementation of SC resulted in numerically higher yields of FCM (41.9 vs. 40.1 kg/d) and ECM (41.8 vs. 40.3 kg/d), and milk fat percentage (3.64 vs. 3.43%). While the MOS diet had no effects on performance compared to the Control, the combination treatment MOS+SC increased milk protein percentage (p<0.05). Also, the MOS supplementation, both alone or in combination with SC, numerically increased milk fat percentage. The SC supplementation increased apparent digestibility of DM and crude protein while the MOS supplementation did not affect digestibility. Concentrations of total volatile fatty acids (VFA) and ruminal pH were similar across treatments. Overall results indicated that supplementation of MOS produced variable and inconsistent effects on rumen metabolism and performance, whereas SC supplementation improved nutrient digestibility and numerically increased FCM and ECM yields, which could not be enhanced by the

Highlights

  • During early lactation, cows experience huge negative energy balance and insufficient dry matter intake (DMI)that may increase the incidence of energy-related metabolic disorders

  • Received September 30, 2008; Accepted February 2, 2009 including mannan, galacto, and fructo-oligosaccharides, which recently have been used as prebiotics in monogastric feeding regimens (Shafey et al, 2001; Yang et al, 2007), may be examined in ruminant diets to test whether or not these compounds act as agents that selectively attach to bacteria and may modify ruminal metabolism

  • Data were analyzed using the MIXED procedure of Animal performance and feed intake Dry matter intake was not affected by supplementation

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Summary

Introduction

Cows experience huge negative energy balance and insufficient dry matter intake (DMI)that may increase the incidence of energy-related metabolic disorders. As achieving maximum potential intake is difficult during this critical stage, a promising approach is to use additives that increase the digestibility of the diet, especially fiber fractions, and increase energy and nutrient supply. Live yeasts are among those additives that have been shown to increase digestibility of fiber and CP (Erasmus et al, 1992; Wohlt et al, 1998) in some but not all (Arambel and Kent, 1990; Wohlt et al, 1991) studies. Received September 30, 2008; Accepted February 2, 2009 including mannan-, galacto-, and fructo-oligosaccharides, which recently have been used as prebiotics in monogastric feeding regimens (Shafey et al, 2001; Yang et al, 2007), may be examined in ruminant diets to test whether or not these compounds act as agents that selectively attach to bacteria and may modify ruminal metabolism. Irreversible attachment of fructo- and mannan-oligosaccharides to pathogens, which thereby reduce the chance of pathogen attachment to intestinal mucosa, has been documented (Sohn et al, 2000)

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