Effect of liraglutide on glucose transporter 4 translocation of mouse skeletal muscle cells

Abstract

Objective To investigate the effects and mechanism of glucagon-likepeptide1(GLP-1) analogue liraglutide on glucose transporter 4 (GLUT4) translocation of mouse skeletal muscle cells. Methods In overexpressing C2C12-GLUT4HA cells, observing the effects and molecular mechanism of liraglutide on GLUT4 translocation. C2C12-GLUT4HA cells were divided into the control group, insulin group(100 nmol/L), liraglutide I group(100 nmol/L), liraglutide II group(1 000 nmol/L), 5-aminoimidazole-4-carboxamide 1-β-D-ribofuranoside (AICAR) (adenosine 5'-monophosphate (AMP)-activated protein kinase agonist)group (2 mmol/L). The amount of GLUT4HA on the cell surface was measured by enzyme-linked immunosorbent assay(ELISA).The phosphorylation of adenosine 5'-monophosphate (AMP)-activated protein kinase(AMPK), protein kinases B (AKT), and GLUT4 expression were tested by western blotting. Statistical analyses were performed using Student's t test or analysis of variance (ANOVA). Results The amount of GLUT4HA on the cell surface in liraglutide groups was more than the amount of GLUT4HA on the cell surface in control group (1.53±0.28 vs 1.41±0.41, F=13.4798,P 0.05). Conclusion This study suggests that Liraglutide may induce GLUT4 translocation of mouse skeletal cells by activation of AMPK. Key words: Liraglutide; Glucose transporter 4 translocation; Adenosine 5'-monophosphate(AMP)-activated protein kinase; Skeleteal musle cell