Effect of lipid raft disruption on TRPV1 receptor activation of trigeminal sensory neurons and transfected cell line

Abstract

Event Abstract Back to Event Effect of lipid raft disruption on TRPV1 receptor activation of trigeminal sensory neurons and transfected cell line Éva Szőke1*, Rita Borzsei1, D. M. Toth2, O. Lengl1, Zsuzsanna Helyes1, Z. Sandor2 and Janos Szolcsanyi1, 2 1 Department of Pharmacology and Pharmacotherapy, University of Pécs, Hungary 2 University of Pécs and Gedeon Richter Plc., Hungary The transient receptor potential vanilloid type 1 (TRPV1), is a nociceptive plasma membrane protein, which can be activated by capsaicin, resiniferatoxin (RTX), low pH, and noxious heat. Since in single patch clamp preparations both RTX and capsaicin opened the same ion channel it has been suggested that allosteric binding of the two agonists to the TRPV1 ion channel-lipid raft complex is responsible for the paradoxical low affinity of capsaicin to the RTX binding site. Lipid rafts are defined as liquid ordered plasma membrane microdomains rich in cholesterol and sphingolipids. The aim of the present study is to analyse the effects of lipid raft disruption by the membrane cholesterol depletor methyl -cyclodextrin (MCD), by sphingomyelinase or sphingolipid synthesis inhibitors like D-PDMP or myriocin. Ratiometric technique of [Ca2+]i measurement on cultured trigeminal cells and radioactive calcium-45 uptake experiments in transfected CHO cells were performed. MCD and sphingomyelinase significantly diminished the intracellular Ca2+ influx induced by capsaicin, but had no significant effect on the Ca2+ uptake caused by RTX. Whereas these caused significant inhibition in TRPV1 receptor activation evoked by capsaicin as well as RTX on cultured trigeminal neurons. D-PDMP and myriocin significantly reduced the intracellular Ca2+ influx induced by both capsaicin and RTX on both experimental objects. These data suggest that in TRPV1 gating mechanisms differences is responses evoked by various stimuli obtained on transfected cell lines and native sensory neurons might be partly related to their differences present in their lipid raft, protein, plasma membrane arrangement. Conference: 12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009. Presentation Type: Poster Presentation Topic: Pathophysiology and neurology - non-degenerative disorders Citation: Szőke É, Borzsei R, Toth DM, Lengl O, Helyes Z, Sandor Z and Szolcsanyi J (2009). Effect of lipid raft disruption on TRPV1 receptor activation of trigeminal sensory neurons and transfected cell line. Front. Syst. Neurosci. Conference Abstract: 12th Meeting of the Hungarian Neuroscience Society. doi: 10.3389/conf.neuro.01.2009.04.241 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 13 Mar 2009; Published Online: 13 Mar 2009. * Correspondence: Éva Szőke, Department of Pharmacology and Pharmacotherapy, University of Pécs, Pecs, Hungary, eva.szoke@aok.pte.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Éva Szőke Rita Borzsei D. M Toth O. Lengl Zsuzsanna Helyes Z. Sandor Janos Szolcsanyi Google Éva Szőke Rita Borzsei D. M Toth O. Lengl Zsuzsanna Helyes Z. Sandor Janos Szolcsanyi Google Scholar Éva Szőke Rita Borzsei D. M Toth O. Lengl Zsuzsanna Helyes Z. Sandor Janos Szolcsanyi PubMed Éva Szőke Rita Borzsei D. M Toth O. Lengl Zsuzsanna Helyes Z. Sandor Janos Szolcsanyi Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.