Abstract

The effects of light intensity, temperature and pH on photosynthetic characters of Haematococcus pluvialis CG-11 were studied by measuring photosynthetic O2 evolution and fluorescence kinetics. The results showed that the saturation light intensity was 109.1 μmol/m2·s of H. pluvialis CG-11, with the maximum photosynthetic oxygen evolution rate of 75.9 μmol O2/mg Chla·h; the range of suitable growth temperature was between 25℃ to 30℃, the maximum photosynthetic rate was obtained at 25℃; the photosynthetic efficiency kept quite high at the pH range of 7.5—8.0,with the maximum photosynthetic oxygen evolution rate of 75.5 μmol O2/mg Chla·h at pH value of 7.5. Under experimental conditions of pH, a similar tendency manifested in the chlorophyll fluorescence kinetics parameters for H. pluvialis, at the pH range of 7.5—8.0, the Fv/ Fm, Fv/ F0, ΦPSⅡand ETR increased following the rising of pH, with their maximum values at pH 7.5, Fv/ Fm and Fv/ F0 decreased significantly over pH 7.5, while the decreasing trend didn’t change obviously in ΦPSⅡ and ETR.

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