Abstract
The present study investigated the effect of sucrose, trehalose and combination of sucrose and trehalose with modified MS medium on in vitro regulation of protocorm-like bodies (PLBs) of Cymbidium devonianum under different quality of lights (white fluorescent tube, green, red and blue LED). As a result of this study, new PLB and shoots were successfully regenerated on modified MS medium under different quality of lights. The highest PLB formation rate (100%) and the highest shoot formation rate (85%) were observed amongst explants cultured on medium supplemented with 10 g/l sucrose + 10 g/l trehalose under green LED. The maximum fresh weight of PLBs, the highest average number of PLBs and shoots were recorded on medium containing 10 g/l sucrose + 10 g/l trehalose under green LED. For plant tissue culture, sucrose is considered an indisputably important carbon and energy source and biosynthesis of trehalose is similar to that of sucrose. The hereby study concluded that the contribution of LED lights, sucrose and trehalose (combined) can induce PLB and shoot formation of Cymbidium devonianum tissue culture without the use of any other plant growth regulator, whereas the green light showed the best formation rate compare with the other studied qualities of lights.
Highlights
Orchids belong to the family Orchidaceae and are one of the largest and most evolved flowering plants
A great (100%) protocorm-like bodies (PLBs) formation rate was observed when PLBs were cultured on modified MS medium under green light-emitting diodes (LED) and 90% PLB formation rate was observed with the other three different sources of lights
The highest number of PLBs (15.1 PLBs/explant) and maximum fresh weight 122.3 mg were observed under green LED
Summary
Orchids belong to the family Orchidaceae and are one of the largest and most evolved flowering plants. Cymbidium devonianum (Lindl.) is an epiphytic orchid, relatively rare and considered a threatened orchid species (Das et al, 2011). This species is used in the breeding programs of many miniature hybrids. In vitro micropropagation techniques are useful for the propagation and maintenance of large numbers of threatened plant species (Dhar et al, 2000). Cymbidium is rapidly turning into a model orchid, if not a model plant, at least for in vitro studies, due to the fine scale nature of experiments conducted on this genus (Teixeira da Silva, 2014). An effective strategy becomes essential to salvage and multiply this species
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