Effect of light quality and tissue origin on phenolic compound accumulation and antioxidant activity in Camellia japonica calli

Abstract

In Camellia japonica callus culture, the effects of light quality on the accumulation of phenolic compounds were examined. To this end, the calli were cultured under three monochrome [white (W), red (R), and blue (B)] and two mixed [red/blue (RB) and red/green/blue (RGB)] light sources. After 4 weeks of culture, fresh and dry callus weights were determined, and phenolic content was analyzed using high-performance liquid chromatography. We also examined the effects of RGB light on calli derived from four origins (leaf: CL; root: CR; petal: CP; and ovary: CO). Total phenolic and flavonoid content and DPPH radical scavenging activity were highest in calli exposed to RB and RGB. Catechin was detected only in calli exposed to mixed light (RB and RGB). Interestingly, red pigmentation development in CL and CR calli after 2 weeks of culture and organogenic calli in CR and CP samples was observed. Furthermore, expression of the phenylalanine ammonia lyase and chalcone synthase genes was found to be higher in the CR line than in other lines, and total phenolic and flavonoid content was also higher in the CR and CP lines. The findings of the present study revealed that the accumulation of phenolics and flavonoids in callus tissue varies depending on light quality, which stimulates cell division, and may thus affect the proliferation and organogenesis of C. japonica callus. We also established that the effects of light on red pigment expression and the accumulation of bioactive compounds are dependent on the tissue origin of callus material.