Abstract
Summary A method was developed to measure the solubilized DNA-dependent RNA polymerase (nucleosidetriphosphate: RNA nucleotidyltransferase, EC 2.7.7.6) activity in pea buds (Pisum sativum, var. Alaska). The method, involving solubilization of enzymes and their partial purification by a step-wise elution via a DEAE-Sephadex column, was used to study the effect of illumination time on enzyme activity. The amounts and proportions of the RNA polymerases I and II were almost constant until 48 h of illumination. Properties of isolated RNA polymerases I and II were not observed to alter during illumination. These results suggest that several factors are involved in photomorphogenesis.
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