Abstract

Chlorella sp. and Monoraphidium sp. were the potential microalgal species for lipid production. This study aimed to investigate different light intensities (40, 200, 400μmolphotonm−2s−1) on physiological changes, photosynthetic carbon partitioning and neutral lipid accumulation in both microalgae. Results suggested that under high light (HL, 400μmolphotonm−2s−1), chlorophyll degraded, protein and carbohydrate content decreased; more carbon allocated into lipid as well as most of intracellular space was occupied by lipid bodies. Moreover, with the lipid accumulation, Fv/Fm decreased and ROS scavenging enzyme increased. Membrane lipid reduced dramatic (29.73–37.97%) to format NL (71.66% of total lipid in Chlorella sp. L1 and 60.65% in Monoraphidium dybowskii Y2). The NL productivity under HL (51.36 and 49.71mgL−1d−1) were more than 3 times of those under LL. Additionally, FAME profiles proved that the useful fatty acid components for biodiesel production were enhanced under HL.

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