Effect of Light Emitting Diode and TiO2-decorated Ag Nanoparticles on Skin Cancer A431 Cell Line

Abstract

Background: Devices based on light-emitting diodes (LEDs) are the newest and safest method for treating a variety of ailments, and dermatologists are now routinely using them. To confirm the effectiveness of this type of treatment, more controlled research are still required. Materials and Methods: A431 Cell line and treatment with blue LED at output light wave intensity 1200-1400 mw/cm. photodynamic therapy (PDT) with TiO2/Ag using human skin cancer cells as experimental model were used. Cell death and a possible mechanism of action were studied. Results: Blue light (wavelength 420–480 nm) exposure caused a rapid and significant decline in viability, which was followed by the death of over half of the cells. The current paper reviews the state of the art in skin LED-based treatment methods. The newest and safest treatment is LED therapy with nanoparticle, the treatment of radioactively irradiated cells with LED causes an increase in cell death. Blue light exposure might open up new possibilities for treating superficial skin cancers in people. Conclusion: The blue light (420–480 nm) of the light-emitting diode had a better anticancer effect on the SCC cell line after 24 hours of incubation. The best results were obtained with a 240-second exposure time in light with a TiO2/Ag concentration of 400 µg/ml.