Abstract
Background. In case of a corneal burn injury, cell transplantation into the damaged area must be performed within the first 12 hours, which makes it impossible to use autologous stem cells. One solution to this problem may be the use of peptides, derived from cultured stem cells in the treatment and prevention of complications in a burn eye disease. Aims: To study the dynamics of corneal tissue repair under the influence of a peptide solution on a corneal thermal burn model.Materials and methods. The study included 20 rabbits (20 eyes) of the gray Chinchilla breed weighing from 2.5 to 3.2 kg with a corneal thermal burn model. Depending on the method of treatment used, the animals were divided in two groups of 10 rabbits (10 eyes). In the experimental group, instillations of a peptide solution were used to treat corneal thermal burns; in the control treatment was carried out with a solution of moxifloxacin and gel “Solcoseryl”. On the 1st, 3rd, 7th, 14th, 30th days in each group, two animals were sacrificed to conduct a morphological study of the cornea. Results. In the experimental group, by the 30th day, according to a histological study, the inflammatory process was completed both on the surface and inside the cornea, with a tendency to restore its normal structure. In the control group of animals, significantly longer periods of corneal recovery and preservation of inflammation, despite the received therapy, were observed.Conclusions. The use of the peptide preparation is promising in the treatment of corneal thermal burn. Further research is needed in this area.
Highlights
In case of a corneal burn injury, cell transplantation into the damaged area must be performed within the first 12 hours, which makes it impossible to use autologous stem cells
One solution to this problem may be the use of peptides
the animals were divided in two groups of 10 rabbits
Summary
In case of a corneal burn injury, cell transplantation into the damaged area must be performed within the first 12 hours, which makes it impossible to use autologous stem cells. При любых повреждениях роговицы ЛСК могут генерировать эпителиальные клетки для заполнения эрозированных областей [1]. В опытной группе для лечения термического ожога роговицы применяли инстилляции раствора пептидов; в контрольной лечение проводилось раствором моксифлоксацина и гелем, состоящим из депротеинизированного диализата телячьей крови. До моделирования термического ожога роговицы для получения препарата пептидов у животных из тазовой кости выделяли стволовые клетки костного мозга под общей анестезией.
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More From: Acta Biomedica Scientifica (East Siberian Biomedical Journal)
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