Abstract
Effect of leptin status on neuroendocrine-reproductive regulation in wistar rats was studied. Ten wistar rats weighing between 170-280g were randomly assigned into two study groups. The animals in Group 1 (the control) received a placebo of 5.0ml distilled water while those in Group two were treated with 100mg insulin/kg body weight of rat via gastric intubation. The experiment lasted for 21 days. One day after the final exposure, the animals were euthanized by inhalation of over dose of chloroform. The brain of each rat was harvested and processed into whole homogenate, and was used for some biochemicals assays (i.e isolation and purification of RNA, reverse transcription polymerase chain reaction (PCR), and leptin assay). The results showed that insulin increased the secretion of leptin, which in turn, reduced feed intake, and energy balance, leading to increased MRNA expression, suggesting that leptin may beinvolved in the control of appetite and maturation of luteneizing hormone secretory axis, which may be associated with development of the neuroendocrine axis (i.e neuroendocrine signal transduction). The study may suggest that leptin may serve as effectors that link mechanism that regulate reproduction and energy balance, thus playing an importantrole in reproduction and energy balance; modulating maternal nutrient partitioning inorder to optimize the provision of nutrients for fetal growth.
Highlights
Leptin is a ploypeptide hormone, produced by adipocytes and trophoblast cells (Masuzaki et al, 1997; Hardie et al, 1997; Holness et al, 1999)
These effects are mediated by interactions between neurotransmitters such as norepinephrine, dopamine, and neuropeptides, including neuropeptide Y (NPY), Gonadotrophin-releasing hormone (GnRH), corticotrophin-releasing factor (CRF), orexin, somastostatin, propiomelanocortin (PoMc), and peripheral hormones that act at the hypothalamus (e.g leptin or insulin) (Schewartz et al, 1996; Erickson et al, 1996; Schewartz et al, 1997; Thorton et al, 1997; Fliers et al, 1998; Hakaanson et al, 1998)
The results showed that expression of ob-RL was higher (P
Summary
Leptin is a ploypeptide hormone, produced by adipocytes and trophoblast cells (Masuzaki et al, 1997; Hardie et al, 1997; Holness et al, 1999) This hormone plays an important role in regulating feed intake, energy balance and reproduction (Himms-Hagen, 1999). The long form leptin receptor (ob-Rl) is localized within the hypothalamus along with several neuropeptides that are involved in regulation of the neuroendocrine axis (Hakaansons et al, 1998; Cassanuva et al, 1999). These effects are mediated by interactions between neurotransmitters such as norepinephrine, dopamine, and neuropeptides, including neuropeptide Y (NPY), Gonadotrophin-releasing hormone (GnRH), corticotrophin-releasing factor (CRF), orexin, somastostatin, propiomelanocortin (PoMc), and peripheral hormones that act at the hypothalamus (e.g leptin or insulin) (Schewartz et al, 1996; Erickson et al, 1996; Schewartz et al, 1997; Thorton et al, 1997; Fliers et al, 1998; Hakaanson et al, 1998)
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