Abstract
Objective To investigate the protective effects of leptin on neonatal seizure in rats by behavioral tests and Bcl2 expression. Methods Twenty Sprague-Dawley(SD) rats were divided into four groups: control group, leptin group, RS group and RS+ leptin group on postnatal day 6(P6), 5 rats in each group. From P6 to P12, repeated neonatal seizure model was induced by flurothyl in RS and RS+ leptin group rats. From P13 to P22, leptin (2 mg·kg-1·day-1) was administered by intraperitoneal injection in leptin group and RS+ leptin group rats. Forelimb hanging test and open field test were implemented on P30.Bcl2 expression was detected by western blot on P34. Results (1) Neurobehavioral tests: the time of forelimb suspension in RS group((7.10±1.02) s)was significantly shorter (P<0.05) than the control group((15.95±6.11)s) and the time of forelimb suspension in RS+ leptin group((13.90±2.64) s)was significant longer (P<0.05) than the RS group.In open field test, the number of horizontal movement in RS group(119.80±28.83)was significantly reduced (P<0.05) than that in the control group(191.00±37.27) and the number of horizontal movement in RS+ leptin group(164.20±26.46) was increased than that in the RS group, but the difference was not significant. The number of modification and stool in RS group was significantly increased (P<0.05) than that in the control group and the number of modification and stool in RS+ leptin group was significantly reduced (P<0.05) than that in the RS group.(2)Western blot: the expression of Bcl2 in RS group (0.24±0.07), leptin group(0.89±0.09)and RS+ leptin group(0.56±0.07)was significantly decreased compared with control group(1.02±0.01) (P<0.05), and the expression of Bcl2 in RS + leptin group was significantly increased compared with RS group (P<0.05). Conclusion Leptin improves the brain injury induced by flurothyl on neonatal rats by Bcl2 signaling pathway. Key words: Leptin; Seizure; Bcl2; Brain injury
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