Abstract
目的：观察Survivin sh RNA、结肠腺瘤息肉易感基因（adenomatous polyposis coli,APC）片段联合对HT-29细胞Survivin表达及细胞增殖的影响.方法：构建Survivin sh RNA慢病毒载体、A P C有效片段慢病毒载体,对H T-29细胞分别采用单慢病毒载体侵染及联合侵染.实验分阴性对照组、空载组、sh RNA组、APC组、sh RNA＋APC联合组,对侵染48 h后的HT-29细胞进行real-time PCR、Western blot及CCK8细胞增殖检测,检测Survivin m RNA、蛋白表达水平及对细胞增殖的影响.结果：（1）sh RNA＋APC联合组与其余各组相比,Survivin m RNA表达量显著下降（P〈0.05）;（2）s h R N A＋A P C联合组与其余各组相比,其Survivin蛋白抑制率明显高于其余各组（P〈0.05）;（3）sh RNA＋APC联合组与其余各组相比,细胞增殖速度低于其余各组（P〈0.05）.结论：Survivin sh RNA与APC片段联合能抑制HT-29细胞内Survivin m RNA及蛋白的表达,同时能够抑制细胞增殖能力,并且优于单个基因侵染.
Published Version
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