Effect of Lentinan on Peyer's patch structure and function in an immunosuppressed mouse model

Abstract

Lentinan (LNT), a polysaccharide isolated from Lentinus edodes, has been shown to stimulate immune response. Despite its widespread use owing to its health benefits, epidemiologic and experimental studies that address the biological activities of LNT after oral administration to animals or humans are rare. In this study, the effects of LNT on the intestinal mucosal immune system of immunosuppressed mice were investigated. The number and size of the Peyer's patches (PPs), proliferation ability of PP lymphocytes, T and B lymphocyte percentage, and T-cell activation proportion, as well as the number of M-like cells in PPs, were determined. The antigen transfer ability of M-like cells and intestinal secretory immunoglobulin A (IgA) levels were also detected. After oral administration of LNT for 7 days, the number of PPs, lymphocytes in PPs, and the level of intestinal soluble IgA in immunosuppressed mice were increased. LNT maintained the percentage of T and B lymphocytes and upregulated the proportion of activated T cells in PPs. Furthermore, the number of M-like cells, which were differentiated from intestinal epithelial cells, and their antigen transfer ability were enhanced. These results indicate that orally administered LNT can improve the immune status of the intestinal mucosa in immunosuppressed mice.