Effect of leaf position on levels of the chloroplast and cytosolic fructose bisphosphatase isozymes in the pea leaf nucleus.

Abstract

Immunocytolocalization experiments indicate that nuclear levels of the pea leaf cytosolic fructose bisphosphatase are higher in leaves located near the base of the plant and lower in expanded leaves at the apex. It seems possible that the cytosolic isozyme in the nucleus has a role in tissue aging. In contrast, there is no indication that leaf position or tissue age affects levels of the chloroplastic enzyme in the nucleus. The density of the chloroplast fructose bisphosphatase is higher in the nucleolus than in the nucleoplasm. Conversely, the density of the cytosolic isozyme is slightly higher in the nucleoplasm. Analysis of double immunolabeling experiments indicates that both isozymes are distributed nonrandomly with respect to DNA, and therefore colocalized with DNA, in the nucleus, and that the chloroplast isozyme is also distributed nonrandomly with respect to DNA in the chloroplast.