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Abstract
the study aimed to follow up the effects of injection lead acetate into pregnant females on the testicular development and semen quality of their male births at puberty time. Forty mature female Swiss Webster mice (8-10) weeks old were divided into two equal groups (experimental and control). After mating with fertile males, the pregnant females injected intraperitoneally with 0.1 mg/kg of body weight of lead acetate from the first day of gestation and continued throughout the gestation period 20 days. While the control groups were injected by the same dose and rout of injection with normal saline. At parturition, numbers of the neonate were recorded. After six weeks the male births only isolated and weighted then mated with normal fertile females to evaluate their fertility through assessing their birth rate, then sacrificed to get their testes; each testis was weighted and the left one was fixed, then histological sections with a thickness of 5 microns were prepared. The epididymis was minced with warm normal saline to get a spermatozoal suspension to evaluate the sperm concentration, sperm motility, and abnormal sperm morphology and sperm viability. Results showed a highly significant decrease in the body and testicular weights of experimental male animals. A highly significant reduction in their birth rate after mating with normal partners was recorded together with a significant inverse changes in all semen parameters.
Highlights
Lead has become widely distributed and mobilized in the environment
The epididymis was put in a small Petri dish containing worm normal saline, organ were minced into tiny pieces with micro surgical scissors until getting a homogenized solution, which contain the spermatozoal suspension incubated at 37Cbefor subjected to sperm function test, including microscopic examination to record the concentration of spermatozoa, sperm motility (Grade A, B, C, D), abnormal sperm morphology and sperm viability by using eosin stain
This passage of lead with what is known about its persistence in body for longer time than other metals [14], may explain all the adverse results recorded in this study including the reduction their ability to fertilize normal females which expressed by the significant decrease in their birth rate compared to that of control group
Summary
Lead has become widely distributed and mobilized in the environment. Human exposure and uptake of this element has increased [1]. كغم من وزن الجسم من محلول خلات/ ملغم0.1مزاوجة ھذه الحیوانات مع ذكور بالغة حقنت الاناث الحوامل بـ
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