Abstract
Proteinase K and extracts of virus-diseased larvae (LX) significantly reduced the in vitro viral titers of the nonoccluded virus (NOV) of the single-enveloped nuclear polyhedrosis virus of Heliothis zea (HzSNPV). Similar treatment of alkali-liberated occuluded virions (LOVAL) increased the viral titer by as much as 2.5 logs. Alkali dissolution of heat treated polyhedral inclusion bodies (PIB) of HzSNPV with sodium carbonate consistently resulted in high titers ranging from 105.3 to 106.9 TCID50/ml.
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