EFFECT OF LACTOBACILLUS PLANTARUM ONU 87 IN MIXTURE WITH AUTOLYSATE OF ERWINIAS ON FORMATION OF TUMORS CAUSED BY RHIZOBIUM RADIOBACTER C 58

Abstract

The aim of the research was to investigate the effect of mixture of Lactobacillus plantarum ONU87 and autolysate of Erwinia carotovora ZM1 cells containing macromolecular bacteriocins and bacteriophages on the pathogenesis of the crown gall. Materials and Methods. As an infectious agent, a strain of Rhizobium radiobacter C58 characterized by its high virulence was chosen. As test-models, carrot roots (Daucuscarota subsp. sativus L.) and plants Kalanchoe daigremontiana Mill were used. Results. The effect of mixture of L. plantarum ONU87 and autolysate of E. carotovora ZM1 on survival of R. radiobacter C58 in vitro has been studied. Amount of viable cells of plant pathogenic bacteria decreased after 4 hours of culturing. Treatment with a mixture of lactobacilli and autolysate of erwinias results in complete inhibition of crown gall pathogenesis on Kalanchoe plants. Treatment of carrot roots with an experimental mixture results in decrease of the amount of infected explants in 14.3%.The mentioned results enabled us to conclude that depending on the sensitivity of the test-objects, treatment with the mixture of L. plantarum ONU87 and autolysate of E. carotovora ZM1 results in complete inhibition of crown gall pathogenesis or decrease of its symptoms.