Abstract
Anoectochilus formosanus (family Orchidaceae) is a perennial herb that contains many bioactive valuable. In this study, the extract efficiency of bioactive compounds from Anoectochilus formosanus Hayata by microwave-assisted extraction (MAE), ultrasonicassisted extraction (UAE) treatments, and lactic fermentation in individual and combined impacts were evaluated through the total phenolic content, total polysaccharide content, and antioxidant activity. The results showed that MAE and UAE treatments and lactic fermentation enhanced the effective extraction of bioactive compounds compared to the control samples. The bioactive compound contents from A. formosanus fluid trend differences in individual treatment factors. However, there was no significant difference between these treatment factors in which the fermentation process requires more time to reach the expected extraction efficiency. The ultrasonic pretreatment combined lactic fermentation process would bring many benefits; first, no need for more the MAE treatment; second, reduce fermentation time; third, bring a probiotic source as well as valuable metabolic products from the lactic fermentation process.
Highlights
Anoectochilus formosanus is a perennial herb that contains many bioactive valuable
The results showed that microwave-assisted extraction (MAE) and ultrasonicassisted extraction (UAE) treatments and lactic fermentation enhanced the effective extraction of bioactive compounds from A. formosanus compared to the control samples
The present study showed that the MAE treatment at 600 W of power in 3 min or the UAE treatment at 25% in 10 min had higher extraction efficiency than control samples (Figure 2)
Summary
Anoectochilus formosanus (family Orchidaceae) is a perennial herb that contains many bioactive valuable. There are about 35 species of Anoectochilus distributed in India, the Himalayas, Southeast Asia, Indonesia, New Caledonia, and Hawaii (Tseng et al, 2006). Many bioactive compounds from A. formosanus were isolated and demonstrated that capable of preventing and against diabetes, antioxidants, and reducing cholesterol (Tang et al, 2018). The result of many studies proved that A. formosanus is a plentiful source material of medicinal compounds extremely important. Du et al (2008) isolated many powerful antioxidants, their derivatives which were determined as glycoside, kinsenoside and acid butanoic. The diarylheptanoid and three flavonoid glucosides are four of the main phenolic compounds in component BuOH of A. formosanus, which is determined with strong antioxidant characteristics (Wang et al, 2002). Studies on the extraction of bioactivity components from A. formosanus is a potential approach
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