Effect of L-arginine and Eugenol on Ram Semen Kinematic Parameters and Post Thawed Fertility Rate after Trans-cervical Artificial Insemination

Abstract

Backgroung: Long term storage of spermatozoa is one of the most desirable tools of assisted reproductive technologies. The purpose of this research was to evaluate the effect of two antioxidants on sperm kinematic parameters after the freezing-thawing process and the post thawed fertility rate after the trans cervical artificial insemination. Methods: Ejaculates from Lacune rams were collected using artificial vagina, were pooled and diluted using based extender (Biladyl, Minitube) and based extender with antioxidants (Eugenol, Arginine). Trans-cervical artificial insemination was performed with frozen seminal material in straws. Result: No significant differences were observed in samples cryopreserved in a base extender and in the base extender supplemented with Arginine. In Eugenol supplemented extender the kinematic parameters were significantly higher for p=0.5 (30.15% progressive motility after thawing) than in the others groups (23.57%, 24.38%). The fertility rate was higher for the straws conserved in extender supplemented with Eugenol (80%) compared with the base extender (55.55%) and the base extender supplemented with Arginine (60%).