Abstract

With the objective of improving the impact of multi-enzymes feed additives on cecal fermentation pattern and rabbit metabolism, sodium bentonite was co-supplemented with “Kemzyme”, a multi-enzyme blend of Kemin Agrifoods Europe. Co-supplementation decreased cecal pH value, increased total VFAs concentration, increased propionates at the expence of acetates and butyrates, increased fermentation efficieny and VFAs utilization. Additionally, co-supplementation increased serum glucose concentration and decreased serum triglycerides and cholesterol concentration. Cecal ammonia nitrogen and serum urea concentration were also decreased by co-supplementation while no change was recorded in serum total proteins concentration. The study therefore, suggested that, coupling bentonite to multi-enzyme feed additives could improve the impact of such enzymes on cecal fermentation pattern and rabbit metablism.

Highlights

  • Data were analyzed by one way analysis of variance (ANOVA) test according to Snedecor and Cochran (1980)

  • Treatment means were compared by the least significance difference (LSD) at 5% level of probability

  • According to Garcia et al (2002), VFAs concentrations could reach a value of up to 99.8 mmol·l-1, depending on rabbit’s age and physiological status as well as food ingredients. It appears that fermentation within the cecum proceeded normally after co-supplementation as VFAs concentrations amounted to 75.8 mmol·l-1

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Summary

Materials and methods

Twenty rabbits of the Newzealand white breed, 12-week old and approximately 1.2 kg body weight were and randomly divided into four groups (5 in each). All rabbits received ad libitum a balanced commercial pellet diet (composition and chemical analysis are shown, 1). Group 1 kept untreated and served as a control. Group 2 (kemzyme group) was supplemented with 0.1% “Kemzyme”, a multi-enzyme blend of Kemin Agrifoods Europe, composed of 3000 μ/g beta-glucanase, 5000 μ/g cellulase, 450 μ/g alfa-amylase and 450 μ/g protease. Group 3 (Bent group) was supplemented with 2% sodium bentonite while group 4 (Enz+Bent group) was supplemented with 0.1% Kemzyme plus 2% sodium bentonite. Doses of supplemented additives were given mixed with the basal ration in the form of pellets. The experiment lasted for 12 weeks during which fresh, clean water was available at all times

Sampling procedures
Analytical Procedures for Samples of Cecal Digesta
The VFAs utilization index
Statistical analysis
Results and discussion
Cecal fermentation traits
Blood biochemical parameters
Conclusion
26.20 Ether extract
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