Abstract
The present study was aimed to study the effect of kaempferol, on the transgenic Drosophila model of Parkinson’s disease. Kaempferol was added in the diet at final concentration of 10, 20, 30 and 40 µM and the effect was studied on various cognitive and oxidative stress markers. The results of the study showed that kaempferol, delayed the loss of climbing ability as well as the activity of PD flies in a dose dependent manner compared to unexposed PD flies. A dose-dependent reduction in oxidative stress markers was also observed. Histopathological examination of fly brains using anti-tyrosine hydroxylase immunostaining has revealed a significant dose-dependent increase in the expression of tyrosine hydroxylase in PD flies exposed to kaempferol. Molecular docking results revealed that kaempferol binds to human alpha synuclein at specific sites that might results in the inhibition of alpha synuclein aggregation and prevents the formation of Lewy bodies.
Highlights
The present study was aimed to study the effect of kaempferol, on the transgenic Drosophila model of Parkinson’s disease
The results of the present study reveal that the kaempferol is potent in reducing the oxidative stress, cognitive dysfunction, and prevents the loss of dopaminergic neurons
Natural antioxidants such as phenols, flavonoids and tannins play an important role in reducing the oxidative stress by scavenging free radicals and reactive oxygen species thereby preventing the protein, lipid and DNA d amage[17,18].The autopsies of the brain of Parkinson’s disease (PD) patients have revealed that oxidative stress is responsible for lipid, proteins and DNA damage along with decreased in the activity of Superoxide dismutases (SODs), catalase and glutathione levels[19,20,21]
Summary
The present study was aimed to study the effect of kaempferol, on the transgenic Drosophila model of Parkinson’s disease. The animal models are helpful in screening and testing of new therapeutic agents for PD5 In this context Drosophila has emerged as an effective model for studying PD pathogenesis[6]. Feany and Bender[7] have developed transgenic Drosophila melanogaster for understanding the mechanisms of PD These PD flies express human α-synuclein, protein aggregation (Lewy bodies formation), decline in dopaminergic neurons and locomotor defects[5]. Kaempferol is a yellow coloured compound commonly found in plants It has a defensive effect against the brain oxidative damage induced by various types of agents[10,11]. Our present study explores the protective potential of kaempferol in transgenic Drosophila model of PD
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
