Effect of JY adjuvant on mucosal immune response to human papillomavirus types 16 and 18 L1 virus-like particles

Abstract

Objective To evaluate the effect of JY adjuvant, which is composed of IL-2 and chitosan, on the immune response for mucosal immunization with human papillomavirus types 16 and 18 L1 virus-like particles (HPV16+ 18 L1 VLP). Methods Mice were immunized three times with HPV16+ 18 L1 VLP in the presence or absence of JY adjuvant by intramuscular and intranasal routes, respectively. Subsequently, experiments were undertaken to detect serum IgG antibody, neutralizing antibody and respiratory tract washes sIgA antibody titers and cellular immune response. Results Following intranasal immunization, serum IgG antibody titers were much higher in HPV16+ 18 L1 VLP with JY adjuvant group than that in VLP without adjuvant group (P<0.01), after intramuscular immunization, serum IgG titers induced by VLP with or without JY adjuvant were the same; following intramuscular immunization, neutralizing antibody titers induced by adjuvant-containing HPV16+ 18 L1 VLP were higher than those by adjuvant-free VLP, following intranasal immunization, only serum neutralizing antibody was detected in adjuvant-containing VLP group; after intranasal immunization, lung washes sIgA concentration were much higher in HPV16+ 18 L1 VLP with JY adjuvant group than that in VLP without adjuvant group (P<0.05); following intranasal and intramuscular immunization, respectively, the number of spot forming cells were much higher in HPV16+ 18 L1 VLP with JY adjuvant group than that in VLP without adjuvant group (P<0.01, P<0.05, respectively). Conclusion JY adjuvant enhanced the cellular, humoral and mocosal immunities induced with HPV16+ 18 L1 VLP by intranasal route, while showed no significant influence of the adjuvant was seen in the group immunized by intramuscular route. Key words: Adjuvants, immaunologic; Papillomvirus, human; Virion; Immunity, mucosal