Abstract
Objective To explore the effect of juxtaposed with another zinc finger gene 1 (JAZF1) on protein kinase B (Akt) and AMP-activated protein kinase (AMPK) signal pathway in 3T3-L1 adipocytes.Methods Recombinant pGenesil-JAZF1shRNA plasmids were constructed.3T3-L1 adipocytes transfected with pGenesil-HK or pGenesil-JAZF1 plasmid were treated with insulin or liraglutide.Glucose uptake was detected by 2-deoxy-3H-glucose.The phosphorylations of Akt and AMPK in 3T3-L1 adipocytes were detected by Western blot.Results Recombinant pGenesil-JAZF1shRNA plasmids was transfected into 3T3-L1 adipocytes and JAZF1 expression was downregulated,resulting in decreased basal and insulin-stimulated glucose uptake by 33.6％ and 38.8％ (both P＜ 0.05).Insulin and liraglutide markedly increased the phosphorylation levels of Akt and AMPK in 3T3-L1 adipocytes (P ＜ 0.05).The downregulation of JAZF1 expression decreased basal and this two hormones-stimauhed phophorylations of Akt and AMPK (all P＜0.05).Conclusion JAZF1 gene may improve glucose metabolism through Akt and AMPK signal pathway. Key words: JAZF1 ; Protein kinase B; AMP-activated protein kinase; Insulin; Liraglutide
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