Abstract
Neutral detergent fiber (NDF) of wheat straw (WS) war ball-milled for 7, 14, 21, and 28 days in a porcelain rotary ball-mill and hydrolyzed by a cellulare for 4 days, and the residue was used for lignin extraction by either diorane or 1 M NaOH. The effects of ball-milling duration (BMD) and extraction procedure on lignin yield and its high-performance size exclusion chromatography (HPSEC) features were examined in this study. Optimal BMD was 14 days, at which 80% and 100% of the permanganate lignin has been extracted by the dioxane and alkali systems, respectively. At a BMD of 14 days, carbohydrate content war 6% and 15% of the alkali lignin (AL) and diorane lignin (DL), respectively
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