Effect of islet amyloid polypeptide (IAPP/amylin) on 2-deoxyglucose uptake in mouse pancreatic acini

Abstract

In order to examine the effect of islet amyloid polypeptide (IAPP/amylin), a product of the pancreatic β cell and a major component of islet amyloid deposits, on exocrine pancreatic function, we studied the effect of rat IAPP amide (IAPP-NH 2) on 2-deoxy- d-glucose (2-DG) uptake in isolated mouse pancreatic acini. Mouse pancreatic acini were preincubated for 80 min with various concentrations of IAPP-NH 2 (1 nM - 1 μM) and [ 3H]2-DG uptake for 20 min was measured. The full effect of IAPP-NH 2 on this function was not immediate but increased linearly with time for up to 80 min of incubation. IAPP-NH 2 caused a dose-dependent stimulation of 2-DG uptake by mouse acini; a detectable effect at 10 nM and a maximal effect at 1 μM. In the presence of 1 μM IAPP-NH 2, 2-DG uptake increased by 69 ± 8% above basal (mean ± SD, n = 6). The results indicate that IAPP-NH 2 stimulates glucose uptake in mouse pancreatic acini, and raise the possibility that IAPP-NH 2 plays some physiological role in the insuloacinar axis in mouse pancreas.