Effect of islet-activating protein (IAP) on contractile responses of rat vas deferens: Evidence for participation of Ni(inhibitory GTP binding regulating protein) in the α2-adrenoceptor-mediated response

Abstract

The effects of islet-activating protein (IAP) on the contractile responses of vas deferens to exogenous norepinephrine (NE) and electric stimulation (ES) were investigated. IAP treatment had no effect on the contractile response to NE. Pretreatment of the vas deferens with IAP (2.0 μg/ml) for 2 h however significantly attenuated the inhibitory effect of clonidine on ES-induced contractile responses, suggesting that feedback inhibition of presynaptic α 2-adrenoceptors is mediated by the Ni subunit of presynaptic adenylate cyclase.