Effect of Ishophloroglucin A, A Component of Ishige okamurae, on Glucose Homeostasis in the Pancreas and Muscle of High Fat Diet-Fed Mice.

Hye-Won Yang,Myeongjoo Son,Seyeon Oh,Bomi Ryu,You-Jin Jeon,Junwon Choi,Kyunghee Byun

doi:10.3390/md17110608

Abstract

Ishophloroglucin A (IPA), a component of Ishige okamurae (IO), was previously evaluated to standardize the antidiabetic potency of IO. However, the potential of IPA as a functional food for diabetes prevention has not yet been evaluated. Here, we investigated if 1.35 mg/kg IPA, which is the equivalent content of IPA in 75 mg/kg IO, improved glucose homeostasis in high-fat diet (HFD)-induced diabetes after 12 weeks of treatment. IPA significantly ameliorated glucose intolerance, reducing fasting glucose levels as well as 2 h glucose levels in HFD mice. In addition, IPA exerted a protective effect on the pancreatic function in HFD mice via pancreatic β-cells and C-peptide. The level of glucose transporter 4 (GLUT4) in the muscles of HFD mice was stimulated by IPA intake. Our results suggested that IPA, which is a component of IO, can improve glucose homeostasis via GLUT4 in the muscles of HFD mice. IO may be used as a functional food for the prevention of diabetes.

Highlights

Seaweeds contain bioactive substances, such as polysaccharides, proteins, lipids, and polyphenols, and have been reported to have nutraceutical and pharmaceutical potential in functional foods [1,2].Ishige okamurae (IO), an edible seaweed, possesses bioactive substances, such as ishophloroglucin A (IPA), diphlorethohydroxycarmalol (DPHC), and fucoxanthin, as well as other secondary metabolites [3].In our previous study, we suggested that Ishophloroglucin A (IPA) could be used to standardize the antidiabetic activity potency of IO extract in vitro [4]
To assess whether the increased metabolic disorders in high-fat diet (HFD) mice were improved by IPA, we investigated the body weight, food intake, and fasting and feeding glucose levels in HFD mice
We further evaluated the maintenance of glucose homeostasis in the blood of IPA can improve glucose tolerance in HFD mice

Summary

Introduction

Seaweeds contain bioactive substances, such as polysaccharides, proteins, lipids, and polyphenols, and have been reported to have nutraceutical and pharmaceutical potential in functional foods [1,2].Ishige okamurae (IO), an edible seaweed, possesses bioactive substances, such as ishophloroglucin A (IPA), diphlorethohydroxycarmalol (DPHC), and fucoxanthin, as well as other secondary metabolites [3].In our previous study, we suggested that IPA could be used to standardize the antidiabetic activity potency of IO extract in vitro [4]. Seaweeds contain bioactive substances, such as polysaccharides, proteins, lipids, and polyphenols, and have been reported to have nutraceutical and pharmaceutical potential in functional foods [1,2]. Ishige okamurae (IO), an edible seaweed, possesses bioactive substances, such as ishophloroglucin A (IPA), diphlorethohydroxycarmalol (DPHC), and fucoxanthin, as well as other secondary metabolites [3]. The use of IPA in functional foods for diabetes prevention in vivo, in high-fat diet (HFD)-fed mice, has not yet been determined. Glucose homeostasis is maintained by the tight regulation of blood glucose by insulin and glucagon [6]. Glucose transporters (GLUT), with substrate specificities that dictate their functional roles, regulate glucose level both outside and inside of the cell [7].

Methods

Results

Conclusion