Abstract
Red blood cell (RBC) storage lesions have been shown to be associated with some adverse reactions; numerous studies have focused on the lesions caused by storage, and few data on the RBC storage lesions caused by prestorage treatments of leucocyte filtration and irradiation. In this study, we examined the changes related with the RBC storage lesions, including 2,3-diphosphatidylglyceric acid (2,3-DPG), pH, free hemoglobin (Hb), supernatant free K+ and Na+ concentration, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH). Along with the increasing storage time, decreases in 2, 3-DPG levels, pH and Na+ concentration, increases in K+ and free Hb concentrations, and significant morphological changes in RBC in all groups were found. The changes in the groups of irradiation, leucocyte filtration and the combined irradiation and leucocyte filtration were more significant than those in the untreated group. Meanwhile, the MCV levels of the three treated groups were significantly lower than those in the untreated group, while the MCH variations were significantly higher. Our results suggest that irradiation and leucocyte filtration before storage may aggravate blood storage lesions.
Highlights
Blood transfusion administration is a necessary and effective method for clinical surgeries, emergency rescue and hematopathy treatment as well
To analyze the red blood cell (RBC) lesions variations caused by various blood operations and storage time, we investigated the influence of irradiation and/or leukoreduction by filtration on blood quality by measuring 2,3-diphosphatidylglyceric acid (2,3-DPG) levels, pH, free hemoglobin (Hb), supernatant free K+ and Na+ concentration, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and RBC morphology
RBC morphological changes during storage RBC morphology was observed during the storage period using confocal laser scanning microscopy and along with increasing storage time, the normal structure of red blood cells disappeared, and displayed with thin sheet morphology and shrinkage, with a large number of pseudopodia, debris and impurities appearing
Summary
Blood transfusion administration is a necessary and effective method for clinical surgeries, emergency rescue and hematopathy treatment as well. The influence of blood storage lesions (oxygen free radicals damage erythrocyte membranes, increasing their brittleness and decreasing the transformation index) on blood transfusion treatment has been more valuable. Blood will develop different degrees of damage during storage at 4uC, which will significantly affect the health of patients. Procedures such as irradiation and leucocyte filtration before storage will lead to different degrees of lesion to red blood cell (RBC) [1,2]. Due to the acceleration by filtration and irradiation, the US Food and Drug Administration (FDA) limited the maximal storage time of irradiated RBC to 28 days [5], and 35 days by state food and drug administration (SFDA) in China
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