Effect of irradiance, temperature and salinity on growth and toxin production by Nodularia spumigena

Abstract

The object of this work was to determine, using a full-factorial experiment, the influence of temperature, irradiance and salinity on growth and hepatotoxin production by Nodularia spumigena, isolated from Lake Alexandrina in the south-east of South Australia. Higher levels of biomass (determined as particulate organic carbon, POC), toxin production and intracellular toxin concentration per mg POC were produced under light limited conditions (30 μmol m−2 s−1) and at salinities equal to or greater than those experienced in Lake Alexandrina. Both highest biomass and total toxin production rates were recorded at temperatures equal to or greater than those of the lake (20 and 30 °C). The temperature at which maximum biomass and toxin production was recorded decreased from 30 °C for cultures grown at 30 μmol m−2 s−1 to 20 °C when grown at 80 μmol m−2 s−1. In contrast, intracellular toxin per mg POC was highest at the lowest growth temperature, 10 °C, at both 30 and 80 μmol m−2 s−1. It appears that the optimum temperature for biosynthetic pathways used in the production of toxin is lower than the optimum temperature for those pathways associated with growth. Intracellular toxin levels were higher in cells cultured at 10 °C/30 μmol m−2 s−1 whereas the majority of the toxin was extracellular in cells grown at 30 °C/30 μmol m−2 s−1. This implies that the highest concentration of toxin in lake water would occur under high temperature and high irradiance conditions. Individual environmental parameters of salinity, irradiance and temperature were all shown to influence growth and toxin production. Notwithstanding, the overall influence of these three parameters on toxin production was mediated through their effect upon growth rate.