Effect of iRoot Fast Set root repair material on the proliferation, migration and differentiation of human dental pulp stem cells in vitro.

Yan Sun,Ya Shen,Ling Zou,Tao Luo,Jun Liu,Markus Haapasalo,Gianpaolo Papaccio

doi:10.1371/journal.pone.0186848

Yan Sun, Ya Shen + Show 5 more

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https://doi.org/10.1371/journal.pone.0186848

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Journal: PloS one	Publication Date: Oct 23, 2017
Citations: 14	License type: CC BY 4.0

Affiliation: Sichuan University, University of British Columbia

Abstract

The present study investigated the effect of iRoot Fast Set root repair material (iRoot FS) on the proliferation, migration and differentiation of human dental pulp stem cells (hDPSCs). The hDPSCs were treated with eluates of iRoot FS at concentrations of 0.2 and 2 mg/mL, referred to as FS0.2 and FS2, respectively, and Biodentine (BD; Septodont, Saint Maur des Faussés, France) eluates at the corresponding concentrations as positive controls. A CCK8 assay was performed to determine cell proliferation. Wound healing and transwell assays were conducted to examine cell migration. Osteogenic differentiation was evaluated based on alkaline phosphatase activity, Alizarin Red S staining and quantitative real-time reverse-transcriptase polymerase chain reaction (qRT-PCR) to analyze the mRNA expression of differentiation gene markers. Cell proliferation was higher in the FS and BD groups than in the blank controls at 3 and 7 days. Moreover, FS0.2 enhanced cell migration and significantly promoted the osteogenic differentiation of hDPSCs. These findings suggested that iRoot FS is a bioactive material that promotes the proliferation, migration and osteogenic differentiation of hDPSCs.

Highlights

Dental pulp exposure commonly results from caries and trauma
Mesenchymal stem cell (MSC) surface markers CD29 and CD44 were expressed at levels higher than 96% in human dental pulp stem cells (hDPSCs) (Fig 1)
At 3 days, cell proliferation was similar in BD0.2, BD2, FS0.2, and FS2

Summary

Introduction

Dental pulp exposure commonly results from caries and trauma. Direct pulp capping (DPC) is the most suitable treatment for this condition. The medicament or material is placed on the exposed pulp to maintain pulp vitality and induce the formation of reparative dentin [1]. The pulp-capping material is an important factor influencing the survival of the pulp [2]. An ideal pulp-capping agent should be easy to use, provide a good seal to dentin, cause little or no irritation to the pulp tissue, promote cell attachment and migration, and stimulate the production of reparative dentin.

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