Effect of Iron Availability on the Growth and Microcystin Content of Natural Populations of Microcystis spp. from Reservoirs in Central Argentina: A Microcosm Experiment Approach

Abstract

The eutrophication of aquatic systems is a problem related to the contribution of excess nutrients—phosphorus (P) and nitrogen (N)—to water bodies, which produces an increase in cyanobacterial blooms. Under eutrophic conditions, P and N concentrations are sufficient for cyanobacteria growth, and some micronutrients are considered to become limiting for population growth. This work aimed to assess the effect of iron on cyanobacteria growth and the content of MCs in natural populations of Microcystis spp. Microcosm setting experiments were carried out with natural samples collected during two bloom events of Microcystis spp., kept under controlled light, temperature and pH conditions. The first bloom sample was exposed to different iron concentrations (400, 700 and 1100 µg Fe·L−1) to determine the optimum concentration for growth. The second was exposed to different iron addition modes (one: T1P, and two pulses: T2P) to imitate the iron increase produced by the downward migration of Microcystis spp. colonies. Our results show that iron is a growth-promoting factor and that its optimal range of concentrations for the growth of Microcystis spp. under the experimental setting conditions is between 700 and 1100 µg Fe·L−1. On the other hand, growth rates were not significantly different between T1P and T2P; thus, different addition modes did not have an effect on growth. Regarding microcystin content, the MC quota in natural populations of Microcystis spp. did not show a clear relationship with the iron supply. This work contributes to the understanding of the underlying factors affecting cyanobacteria bloom formation and the production of MCs, which in turn would impact the development of management strategies to control cyanobacteria blooms.