Effect of IQGAP1 Knockdown on Homotypic Cell-Cell Adhesion of Esophageal Carcinoma Cells

Abstract

Objective:To investigate the effect of IQGAP1 knockdown by RNA interference(RNAi) on homotypic cell-cell adhesion of esophageal carcinoma(EC) cells.Method:Western blot analysis was adopted to detect the protein expression of IQGAP1 in KYSE150 and EC9706 cells.Slow aggregation assay and cell dissociation assay were performed to detect the homotypic cell-cell adhesion properties of tumor cells.Further,KYSE150 and EC9706 cells were transfected with RNAi plasmid for targeting IQGAP1 gene or negative control,and then the cell lines of IQGAP1 knockdown were screened with G418.Level of IQGAP1 protein in interference group and control group was measured by Western blot,and the changes of homotypic cell-cell adhesion were observed by slow aggregation assay and cell dissociation assay.Result:Compared with EC9706 cells,level of IQGAP1 protein in KYSE150 cells was lower,while the homotypic cell-cell adhesion in KYSE150 cells was stronger.Further,Western blotting analysis showed that IQGAP1 protein expression was efficiently downregulated by stable transfection wiui IQGAP1 siRNA expression vector.The homotypic cell-cell adhesion was obviously enhanced by IQGAP1 knockdown.Conclusion:These data suggest that downregulation of IQGAP1 expression could significantly enhance homotypic cell-cell adhesion and decrease malignant phenotype of EC cells.