Abstract
Purpose:CDK1A is one of the most important genes that have different key roles in cell lines. This gene has several transcript variants. Investigating of expression of each one actually can be so important because any one of them may have a separate unknown role in cancer cells so can be used to increase therapeutic efficacy. Methods:A549, MDA–MB–231 and Hek–AD cell lines were used in this study. Firstly, three primers for variants of p21 gene were designed by Snapgene and BLAST software. Secondly, the variants expression was checked for each cell lines by RT–qPCR technique, separately. Then the variants that expressed in the cells were selected for more investigation. Finally 2 Gy irradiation was used to evaluate the effect of that on variants expression. Results:The results show that for all cell lines, primer num1 and 3 expressed before any stimuli. After irradiation, for MDA–MB–231 and A549, the expression of primer num3 was decreased, while for Hek–AD no change was observed. The primer num1 expression after the irradiation was different for the cells, V1 expression was decreased in A549 by fold of 0.03 while expression of this for MDA–MB–231 cells was not changed after 2Gy irradiation. Conclusion:It is very necessary to pay attention to the function of each splice variant as well as the response to external stimuli. Understanding the role of each variant in a gene is critical and researchers can use that to improve radiotherapy as well.
Highlights
The generation of transcript variants by alternative splicing is a common process in human gene expression; >70% of human genes are known to express transcript variants (Johnson et al, 2003)
The primer num1 expression after the irradiation was different for the cells, V1 expression was decreased in A549 by fold of 0.03 while expression of this for MDA–MB–231 cells was not changed after 2Gy irradiation
We investigated the changes in the expression of p21 variants in three different cell lines followed by 2Gy irradiation
Summary
The generation of transcript variants by alternative splicing is a common process in human gene expression; >70% of human genes are known to express transcript variants (Johnson et al, 2003). Various studies have shown that the transcript variants of a gene may have different and even opposing roles, and alternative splicing is known to be a key factor in cancer progression. Bcl-x, which is associated with cell survival/apoptosis, has two isoforms, Bcl-xL and Bcl-xS. Transcripts V4 and V1 were significantly upregulated but only transcript V4 upregulation was associated with poor recurrence-free survival in The Cancer Genome Atlas data (Xiao et al, 2020). Splice variant (sv) AC3‐33 inhibited MCF‐7 cell proliferation. SvAC3‐33 knockdown by RNA interference was shown to have the opposite effect by repressing the cell cycle progression of breast cancer cells (Yuan et al, 2020)
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